Zhang Yan, Li Xiang, Qiao Jian
Institute of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zhonghua Yi Xue Za Zhi. 2007 Oct 23;87(39):2745-9.
To determine if neurofilament protein light (NF-L) and anti-NF-L antibody can be used as biologic markers in diagnosing multiple sclerosis (MS) and assessing prognosis thereof.
Samples of cerebrospinal fluid (CSF) and serum were corrected from 63 MS patients, 15 patients with neuromyelitis optica (ONM), 31 patients with other inflammatory neurological disease (OIND), 18 patients with noninflammatory neurological disease (NIND), and 46 neurological normal controls (NC group), all age- and sex- matched. ELISA was used to detect the concentrations of NF-L, anti-NF-L antibody, anti-myelin basic protein (MBP) antibody, and anti-myelin oligo-dendroglia glycoprotein (MOG) antibody in serum and cerebral spinal fluid. And the IgG level was tested.
The CSF levels of NF-L of the MS, ONM, OIND, and NIND groups were 26 ng/L (33), 20 ng/L (92), 19 ng/L (82), and 30 ng/L (194) respectively, without significant differences among these different groups, but all significantly higher than that of the NC group (10 ng/L, all P < 0.01). No NF-L was detected in the serum specimens of all groups. The CSF NF-L antibody level of the MS group was significantly lower than that of the OIND group (P < 0.01), and significantly higher than those of the NIND and NC groups (both P < 0.05). The CSF anti-NF-L antibody level of the MS patients was positively correlated with the MBP antibody level (r = 0.784, P < 0.01) and the IgG level (r = 0.675, P < 0.01).
Neither the NF-L nor the anti-NF-L antibody can be used as reliable biological markers for the diagnosis of MS. Strongly increased levels of NF-L were observed in patients with MS, ONM, OIND, and NIND, suggesting the occurrence of axonal injury in these conditions. Inflammation may not lead to the generation of anti-NF-L antibody which links to the axonal injury.
确定神经丝轻链蛋白（NF-L）和抗NF-L抗体是否可作为诊断多发性硬化症（MS）及其评估预后的生物学标志物。
收集63例MS患者、15例视神经脊髓炎（ONM）患者、31例其他炎性神经系统疾病（OIND）患者、18例非炎性神经系统疾病（NIND）患者及46例神经功能正常对照者（NC组）的脑脊液（CSF）和血清样本，所有受试者年龄和性别匹配。采用酶联免疫吸附测定（ELISA）法检测血清和脑脊液中NF-L、抗NF-L抗体、抗髓鞘碱性蛋白（MBP）抗体及抗髓鞘少突胶质细胞糖蛋白（MOG）抗体的浓度，并检测IgG水平。
MS组、ONM组、OIND组和NIND组CSF中NF-L水平分别为26 ng/L（33）、20 ng/L（92）、19 ng/L（82）和30 ng/L（194），不同组间差异无统计学意义，但均显著高于NC组（10 ng/L，均P < 0.01）。所有组血清标本中均未检测到NF-L。MS组CSF中NF-L抗体水平显著低于OIND组（P < 0.01），显著高于NIND组和NC组（均P < 0.05）。MS患者CSF中抗NF-L抗体水平与MBP抗体水平呈正相关（r = 0.784，P < 0.01），与IgG水平呈正相关（r = 0.675，P < 0.01）。
NF-L和抗NF-L抗体均不能作为诊断MS的可靠生物学标志物。在MS、ONM、OIND和NIND患者中均观察到NF-L水平显著升高，提示这些疾病均存在轴突损伤。炎症可能不会导致与轴突损伤相关的抗NF-L抗体的产生。
