Watanabe Masahito, Mitani Nanako, Ishii Naoaki, Miki Keizaburo
Department of Cell Biology, Japanese Institute of Pearl Science, 4-28 Amanuma, Hiratsuka, Kanagawa 254-0031, Japan.
Mutat Res. 2005 Feb 15;570(1):71-80. doi: 10.1016/j.mrfmmm.2004.10.005.
A novel mutant gene, bis-1 (bisphenol A sensitive) has been isolated in the nematode, Caenorhabditis elegans, that affects the response to endocrine disrupting chemicals (EDC). The bis-1(nx3) allele is hypersensitive to bisphenol A (BPA), is allelic to a collagen gene (col-121), and is expressed in hypodermal cells. Among the collagen mutants so far studied, bis-1(nx3), dpy-2(e8), dpy-7(e88) and dpy-10(e128) showed BPA sensitivity. The isolated mutant may work as a useful tool for the assay of EDC toxicity since the physiological effect of the collagen mutation (glycine substitution) indicates an increased sensitivity to BPA.
在秀丽隐杆线虫中分离出一种新的突变基因bis-1（双酚A敏感基因），它影响对内分泌干扰化学物质（EDC）的反应。bis-1(nx3)等位基因对双酚A（BPA）高度敏感，与一个胶原蛋白基因（col-121）等位，且在皮下细胞中表达。在目前研究的胶原蛋白突变体中，bis-1(nx3)、dpy-2(e8)、dpy-7(e88)和dpy-10(e128)表现出对BPA敏感。由于胶原蛋白突变（甘氨酸替代）的生理效应表明对BPA的敏感性增加，因此分离出的突变体可能作为检测EDC毒性的有用工具。
