Rashtchian Sadaf, Youssef Khaled, Rezai Pouya, Tabatabaei Nima
Mechanical Engineering Department, Lassonde School of Engineering, York University, Toronto, Canada.
Biomed Opt Express. 2021 May 25;12(6):3607-3618. doi: 10.1364/BOE.427685. eCollection 2021 Jun 1.
() is an optically transparent nematode that shares many gene orthologs and homologs with humans. are widely used in large populations for genetic studies relevant to human biology and disease. Success of such studies frequently relies on the ability to image structure at high-resolution and high-speed. In this manuscript, we report on the feasibility and suitability of a high-speed variant of reflectance confocal microscopy, known as spectrally encoded confocal microscopy (SECM), for label-free imaging of The developed system utilizes near-infrared illumination in conjunction with refractive and diffractive optics to instantaneously image a confocal image line at a speed of up to 147 kHz with lateral and axial resolutions of 2µm and 10µm, respectively. Our imaging results from wild-type and four mutant strains (MT2124, MT1082, CB61, and CB648) demonstrate the ability of SECM in revealing the overall geometry, key internal organs, and mutation-induced structural variations, opening the door for downstream integration of SECM in microfluidic platforms for high throughput structural imaging of .
（某线虫名称）是一种光学透明的线虫，与人类共享许多基因直系同源物和同源物。（该线虫）被广泛应用于大量群体中，用于开展与人类生物学和疾病相关的遗传学研究。此类研究的成功常常依赖于以高分辨率和高速对（该线虫）结构进行成像的能力。在本手稿中，我们报告了一种称为光谱编码共聚焦显微镜（SECM）的反射式共聚焦显微镜高速变体用于（该线虫）无标记成像的可行性和适用性。所开发的系统利用近红外照明结合折射和衍射光学元件，以高达147kHz的速度即时成像一条共聚焦图像线，横向分辨率和轴向分辨率分别为2μm和10μm。我们对野生型（该线虫）和四种突变株（MT2124、MT1082、CB61和CB648）的成像结果证明了SECM能够揭示整体几何形状、关键内部器官以及突变引起的结构变化，为将SECM集成到微流控平台以进行（该线虫）的高通量结构成像打开了大门。
