Fernandes Priscilla H, Kanuri Manorama, Nechev Lubomir V, Harris Thomas M, Lloyd R Stephen
Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA.
Environ Mol Mutagen. 2005 Jun;45(5):455-9. doi: 10.1002/em.20117.
Vinyl chloride and crotonaldehyde are known mutagens and carcinogens that, through their reaction with DNA, form specific deoxyguanosine adducts. To investigate the mutagenic potential of a subset of the possible deoxyguanosine lesions, site-specific adducts of vinyl chloride and crotonaldehyde were synthesized, inserted into a shuttle vector, and replicated in mammalian cells. Mutation yields of the DNA adducts of vinyl chloride and crotonaldehyde were found to be 2% and 5-6%, respectively, thus suggesting that these adducts could contribute to the overall genotoxicity and carcinogenicity associated with exposure to these chemicals.
氯乙烯和巴豆醛是已知的诱变剂和致癌物，它们通过与DNA反应形成特定的脱氧鸟苷加合物。为了研究一部分可能的脱氧鸟苷损伤的诱变潜力，合成了氯乙烯和巴豆醛的位点特异性加合物，将其插入穿梭载体，并在哺乳动物细胞中复制。结果发现，氯乙烯和巴豆醛的DNA加合物的突变率分别为2%和5 - 6%，这表明这些加合物可能会导致与接触这些化学物质相关的整体遗传毒性和致癌性。
