人肝细胞在固定有RGD的血浆处理膜上培养时的生物转化及肝脏特异性功能

Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes.

作者信息

De Bartolo Loredana, Morelli Sabrina, Lopez Linda C, Giorno Lidietta, Campana Carla, Salerno Simona, Rende Maria, Favia Pietro, Detomaso Loredana, Gristina Roberto, d'Agostino Riccardo, Drioli Enrico

机构信息

Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci cubo 17/C, 87030 Rende (CS), Italy.

出版信息

Biomaterials. 2005 Jul;26(21):4432-41. doi: 10.1016/j.biomaterials.2004.11.009.

DOI:10.1016/j.biomaterials.2004.11.009

PMID:15701372

Abstract

In this paper we report on the metabolic response of human hepatocytes grown on polyethersulfone membranes surface modified with a plasma-deposited acrylic acid coating and RGD peptide covalently immobilized through a "spacer arm" molecule. The modified surfaces were characterized by means of X-ray photoelectron spectroscopy and water contact angle measurements. The performance of modified and unmodified membranes was evaluated by assessing the expression of liver specific and biotransformation functions of human hepatocytes. Diclofenac, a non-steroidal anti-inflammatory drug, was used to investigate the biotransformation functions. Surface-modified membranes elicit specific cellular responses and induce hepatocytes to enhance the synthesis rate of albumin and urea, particularly in the presence of diclofenac. Also the biotransformation functions were expressed at high levels.

摘要

在本文中，我们报告了在聚醚砜膜表面生长的人肝细胞的代谢反应，该聚醚砜膜表面经等离子体沉积丙烯酸涂层修饰，并通过“间隔臂”分子共价固定了RGD肽。通过X射线光电子能谱和水接触角测量对修饰后的表面进行了表征。通过评估人肝细胞的肝脏特异性和生物转化功能的表达来评价修饰和未修饰膜的性能。使用双氯芬酸（一种非甾体抗炎药）来研究生物转化功能。表面修饰的膜引发特定的细胞反应，并诱导肝细胞提高白蛋白和尿素的合成速率，特别是在双氯芬酸存在的情况下。生物转化功能也高水平表达。