De Bartolo Loredana, Morelli Sabrina, Rende Maria, Salerno Simona, Giorno Lidietta, Lopez Linda C, Favia Pietro, d'Agostino Riccardo, Drioli Enrico
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P Bucci cubo 17/C, 87030 Rende (CS), Italy.
J Nanosci Nanotechnol. 2006 Aug;6(8):2344-53. doi: 10.1166/jnn.2006.514.
New strategies aimed to surface modification of polymeric membranes are crucial to optimise cell-biomaterial interactions in vivo and in vitro biohybrid systems. In this paper, we investigated the surface modification of Polyethersulfone (PES) membranes by plasma polymerisation of acrylic acid monomers (PES-pdAA) and by immobilization of galactonic acid through a hydrophilic "spacer arm" molecule (PES-pdAA-SA-GAL). The modification steps were characterised by high resolution X-ray photoelectron spectroscopy. The performance of modified and unmodified membranes was evaluated by assessing the expression of liver specific biotransformation functions of pig and human hepatocytes. Human liver cells cultured on PES-pdAA-SA-GAL membranes displayed an enhanced albumin production, urea synthesis and protein secretion for 24 days of culture. The immobilisation of galactose derivative units on the membrane allowed specific interactions with hepatocytes biomimicking the cellular microenvironment and produced an improvement of the long-term maintenance and differentiation of human hepatocytes.
旨在对聚合物膜进行表面改性的新策略对于优化体内和体外生物杂交系统中的细胞-生物材料相互作用至关重要。在本文中，我们研究了通过丙烯酸单体的等离子体聚合（PES-pdAA）以及通过亲水性“间隔臂”分子固定半乳糖酸（PES-pdAA-SA-GAL）对聚醚砜（PES）膜进行表面改性。改性步骤通过高分辨率X射线光电子能谱进行表征。通过评估猪和人肝细胞的肝脏特异性生物转化功能的表达来评价改性膜和未改性膜的性能。在PES-pdAA-SA-GAL膜上培养的人肝细胞在24天的培养中显示出白蛋白产生、尿素合成和蛋白质分泌增强。膜上半乳糖衍生物单元的固定允许与肝细胞进行特异性相互作用，模拟细胞微环境，并改善了人肝细胞的长期维持和分化。
