Uttamchandani Mahesh, Walsh Daniel P, Yao Shao Q, Chang Young-Tae
Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
Curr Opin Chem Biol. 2005 Feb;9(1):4-13. doi: 10.1016/j.cbpa.2004.12.005.
Directed or exploratory drug development programs constantly seek robust screening platforms for the high fidelity identification and validation of potential targets. Small-molecule microarrays (SMMs) have risen to this call by elegantly forging the capability of combinatorial chemistry in producing myriad compounds with the powerful throughput afforded by microarrays. This synergism offers scientists a versatile tool for rapid compound analysis and discovery. Microarrays of small molecules have already been successfully applied in important areas ranging from protein profiling to the discovery of therapeutic leads. Recent interesting developments towards improved immobilization strategies and library creation methods, together with novel advances herein described, have set the stage for SMMs to take on wider and more routine applications in academia and industry. As a rapidly maturing technology, SMMs pave the way forward in high-throughput exploration, both in the identification of biologically significant natural and synthetic small molecules and in harnessing their vast potential towards medicinal and diagnostic applications.
定向或探索性药物研发项目一直在寻找强大的筛选平台，以实现对潜在靶点的高保真识别和验证。小分子微阵列（SMMs）响应了这一需求，它巧妙地将组合化学生成无数化合物的能力与微阵列强大的通量相结合。这种协同作用为科学家提供了一种用于快速化合物分析和发现的通用工具。小分子微阵列已成功应用于从蛋白质分析到治疗先导物发现等重要领域。近期在改进固定化策略和文库创建方法方面的有趣进展，以及本文所述的新进展，为SMMs在学术界和工业界获得更广泛和常规的应用奠定了基础。作为一项迅速成熟的技术，SMMs在高通量探索方面开辟了道路，既有助于识别具有生物学意义的天然和合成小分子，也有助于挖掘它们在医药和诊断应用方面的巨大潜力。
