Nicholson Rebecca L, Welch Martin, Ladlow Mark, Spring David R
Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, United Kingdom.
ACS Chem Biol. 2007 Jan 23;2(1):24-30. doi: 10.1021/cb600321j.
Cell-permeable small molecules can be used to modulate protein function selectively, rapidly, reversibly, and conditionally with temporal and quantitative control in biological systems. The identification of these chemical probes can require the screening of large numbers of small molecules. With the advent of new technologies, small-molecule high-throughput screening is widely available. This Review focuses on the emerging technologies of microarray screening platforms and high-content screening formats.
细胞可渗透的小分子可用于在生物系统中选择性、快速、可逆且有条件地调节蛋白质功能，并进行时间和定量控制。这些化学探针的鉴定可能需要筛选大量小分子。随着新技术的出现，小分子高通量筛选已广泛可用。本综述重点关注微阵列筛选平台和高内涵筛选形式的新兴技术。
