Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, Madrid, Spain.
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India.
Methods Mol Biol. 2021;2344:3-6. doi: 10.1007/978-1-0716-1562-1_1.
As we approach the twentieth anniversary of completing the international Human Genome Project, the next (and arguably most significant) frontier in biology consists of functionally understanding the proteins, which are encoded by the genome and play a crucial role in all of biology and medicine. To accomplish this challenge, different proteomics strategies must be devised to examine the activities of gene products (proteins) at scale. Among them, protein microarrays have been used to accomplish a wide variety of investigations such as examining the binding of proteins and proteoforms to DNA, small molecules, and other proteins; characterizing humoral immune responses in health and disease; evaluating allergenic proteins; and profiling protein patterns as candidate disease-specific biomarkers. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers involved in the field of protein microarrays provide concise descriptions of the methodologies that they currently use to fabricate microarrays and how they apply them to analyze protein interactions and responses of proteins to dissect human disease.
随着我们即将迎来完成国际人类基因组计划二十周年，生物学的下一个（可以说是最重要的）前沿领域包括对基因组编码的蛋白质进行功能理解，这些蛋白质在生物学和医学中都起着至关重要的作用。为了完成这一挑战，必须设计不同的蛋白质组学策略来大规模研究基因产物（蛋白质）的活性。其中，蛋白质微阵列已被用于完成各种研究，如检查蛋白质和蛋白质变体与 DNA、小分子和其他蛋白质的结合；在健康和疾病中描述体液免疫反应；评估过敏原蛋白；以及分析候选疾病特异性生物标志物的蛋白质图谱。在《疾病分析的蛋白质微阵列：方法与方案》一书中，参与蛋白质微阵列领域的专家研究人员简明描述了他们目前用于制作微阵列的方法，以及他们如何应用这些方法来分析蛋白质相互作用和蛋白质对人类疾病的反应。
