[大孔树脂法纯化沙棘籽渣总黄酮的研究]
[Study on purification of total flavonoids from seed residue of Hippophae rhamnoides with macroporous resin].
作者信息
Cao Qun-hua, Qu Wei-jing, Li Jia-gui, Deng Yun-xia
机构信息
Life Sciences School of East China Normal University, Shanghai, 200062, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2004 Mar;29(3):225-8.
OBJECTIVE
To study the conditions and parameters of purifying total flavonoids from seed residue of H. rhamnoides.
METHOD
Absorption capacity of three resins for total flavonoids was compared. With the yield and purity as indexes, the process of absorbing and purifying total flavonoids from seed residue with D101 macroporous resin absorbent was selected by orthogonal design.
RESULT
The D101 resin was the best of the three resins and its absorption capacity was determined to be 63.00 mg x g(-1) wet resin. The optimum process condition was 30% ethanol as eluting solvent, wet resin vs crude herbs: 2:1, diameter vs height: 1:10, eluting solvent vs crude herbs: 10:1, absorbing time for 3 h. The yield of total flavonoids from seeds residue of H. rhamnoides by this process was 2.39%, and the purity reached 64.81%.
CONCLUSION
The process is simple and convenient and the regeneration of resin is easy. So this method of purification is advisable.
目的
研究从沙棘籽渣中纯化总黄酮的条件及参数。
方法
比较三种树脂对总黄酮的吸附能力。以得率和纯度为指标，采用正交设计法筛选D101大孔树脂吸附剂从沙棘籽渣中吸附纯化总黄酮的工艺。
结果
D101树脂是三种树脂中最佳的，其吸附量为63.00 mg·g⁻¹湿树脂。最佳工艺条件为：以30%乙醇为洗脱剂，湿树脂与药材比例为2:1，柱径与柱高比例为1:10，洗脱剂与药材比例为10:1，吸附时间为3 h。该工艺从沙棘籽渣中提取总黄酮的得率为2.39%，纯度达64.81%。
结论
该工艺简便，树脂易再生。因此该纯化方法可取。
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