[Technique of macroporous resin for tartary buckwheat total flavonoids].

OBJECTIVE

To investigatre appropriate macroporous resins and the optimal technological parameters of the purification process of tartary buckwheat total flavonoids.

METHOD

Static and dynamic adsorption-desorption methods were adopted, and evaluated for separating efficiency by macroporous resins absorption rates, desorption rates.

RESULT

The DM -2 macroporous resin had the best separating efficiency. The best absorbed condition is H2O as solvent of extracts, diameter vs height: 1: 10, pH 3 - 4, absorption power: 3.0 mL x min(-1). The optimum adsorption condition is the volume of 50% ethanol 80 mL (approximately 5 BV (resin bed volume) as eluting solvent, pH 8, the desorption power: 3.0 mL x min(-1).

CONCLUSION

This technology is simple, quick and cost-effective, which is suitable for industrialization.