Yu Zhi-Feng, Wang Min
College of Food Science and Engineering, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China.
Zhongguo Zhong Yao Za Zhi. 2007 Apr;32(7):585-9.
To investigatre appropriate macroporous resins and the optimal technological parameters of the purification process of tartary buckwheat total flavonoids.
Static and dynamic adsorption-desorption methods were adopted, and evaluated for separating efficiency by macroporous resins absorption rates, desorption rates.
The DM -2 macroporous resin had the best separating efficiency. The best absorbed condition is H2O as solvent of extracts, diameter vs height: 1: 10, pH 3 - 4, absorption power: 3.0 mL x min(-1). The optimum adsorption condition is the volume of 50% ethanol 80 mL (approximately 5 BV (resin bed volume) as eluting solvent, pH 8, the desorption power: 3.0 mL x min(-1).
This technology is simple, quick and cost-effective, which is suitable for industrialization.
研究适宜的大孔树脂及苦荞总黄酮纯化工艺的最佳工艺参数。
采用静态和动态吸附-解吸方法，通过大孔树脂吸附率、解吸率评价分离效果。
DM-2大孔树脂分离效果最佳。最佳吸附条件是以水为提取物溶剂，直径与高度比为1:10，pH值3 - 4，吸附流速：3.0 mL×min⁻¹。最佳解吸条件是以50%乙醇80 mL（约5倍树脂床体积）为洗脱溶剂，pH值8，解吸流速：3.0 mL×min⁻¹。
该工艺简单、快速且成本效益高，适合工业化生产。
