[大孔吸附树脂分离纯化菝葜总黄酮的研究]
[Study on separation and purification of total flavones from Smilax china by macroporous absorption resin].
作者信息
Li Wen-xia, Yan Yan, Ye Xiao-chuan, Yang Xiang-liang
机构信息
Department of Chemistry, Wuhan 430074, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Jul;32(13):1292-5.
OBJECTIVE
To investigate the technological parameters of the purification process of total flavones from Smilax china with macroporous absorption resin.
METHOD
The technical process for purification of total flavones with the optimum macroporous absorption resin was screened by yield of total flavones product.
RESULT
The D140 macroporous absorption resin had the best separating efficiency when the flavones content in the liquid was 0.5 g x mL(-1) equivalent to raw material, the volume of drug 18 BV (resin bad volume) with the adsorption-power 2 BV x h(-1), and the volume of 60% (mL x mL(-1)) ethanol as eluant 5-10 BV (resin bad volume) with desorption-power 1 BV x h(-1). The obtained flavones product has total flavones recovery rate of 84.72%.
CONCLUSION
The treatment of regenerated resin is easy, this method is advisable.
目的
研究大孔吸附树脂纯化菝葜总黄酮的工艺参数。
方法
以总黄酮产品得率为指标，筛选出最佳大孔吸附树脂纯化总黄酮的工艺过程。
结果
当液体中黄酮含量为0.5 g·mL⁻¹（相当于原料），药液体积为18 BV（树脂床体积），吸附流速为2 BV·h⁻¹，以60%（mL·mL⁻¹）乙醇为洗脱剂，洗脱体积为5 - 10 BV（树脂床体积），解吸流速为1 BV·h⁻¹时，D140大孔吸附树脂分离效果最佳。所得黄酮产品总黄酮回收率为84.72%。
结论
再生树脂处理简便，该方法可行。
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