Margis R, Pinheiro-Margis M, da Silva L C, Borojevic R
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brazil.
Int J Exp Pathol. 1992 Apr;73(2):125-35.
We have studied the effect of retinol on an established murine cell line (GRX), representative of liver connective tissue cells. This cell line has myofibroblast characteristics; under retinol treatment it is induced into the lipocyte (Ito-cell) phenotype. Retinol decreased the proliferation rate in the entire cell population. It increased cell adherence to the substrate, which was correlated with the increased secretion of fibronectin. Collagen secretion was specifically decreased, whilst the total protein secretion remained stable. Heparan sulphate was decreased in the pericellular compartment, but other glycosaminoglycans were not affected by retinol treatment. Modulations of pericellular components induced by retinol may alter the relations among liver mesenchymal cells, and may be related to vitamin-A-induced modifications of the homoeostasis of hepatic connective tissue and hepatic fibrosis.
我们研究了视黄醇对一种已建立的鼠细胞系（GRX）的影响，该细胞系代表肝结缔组织细胞。这种细胞系具有肌成纤维细胞特征；在视黄醇处理下，它被诱导为脂肪细胞（伊托细胞）表型。视黄醇降低了整个细胞群体的增殖率。它增加了细胞对底物的黏附，这与纤连蛋白分泌增加相关。胶原蛋白分泌特异性减少，而总蛋白分泌保持稳定。细胞周围隔室中的硫酸乙酰肝素减少，但其他糖胺聚糖不受视黄醇处理的影响。视黄醇诱导的细胞周围成分的调节可能会改变肝间充质细胞之间的关系，并且可能与维生素A诱导的肝结缔组织稳态和肝纤维化的改变有关。
