高效液相色谱法测定蜜桶花颗粒及蜜桶花中紫茎女贞苷的含量

[Determination of acteoside in Mitonghua particles and Brandisia hancei by high performance liquid chromatography].

作者信息

Ni Wei, Zhou Lingyun, Yuan Minhui, He Yunping, Chen Changxiang

机构信息

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, The Chinese Academy of Sciences, Kunming 650204, China.

出版信息

Se Pu. 2004 May;22(3):260-2.

PMID:15712913

Abstract

A high performance liquid chromatographic method was established for the determination of acteoside in Mitonghua particles and Brandisia hancei. The results showed that acteoside can be extracted more efficiently with butanol than with water. Therefore, both Mitonghua particles and Brandisia hancei were extracted with butanol. The extracts were analysed on an RP-18 column using a mixture of CH3OH-0.5% aqueous HAc (42:58, v/v) as the mobile phase with UV detection at 334 nm. A good linear response was observed within the range of 0.029-2.038 microg of analytes (r = 0.9998). The recoveries were 90.2%-93.8% and the relative standard deviation was 0.34% (n = 5). This rapid, simple, accurate, and reproducible method is proposed for the quality control of Mitonghua particles.

摘要

建立了一种高效液相色谱法用于测定蜜桶花颗粒和小花龙吐珠中毛蕊花糖苷的含量。结果表明，与水相比，用正丁醇能更有效地提取毛蕊花糖苷。因此，蜜桶花颗粒和小花龙吐珠均用正丁醇进行提取。提取物在RP - 18柱上进行分析，以甲醇 - 0.5%醋酸水溶液（42:58，v/v）的混合液作为流动相，在334 nm波长处进行紫外检测。在0.029 - 2.038 μg分析物范围内观察到良好的线性响应（r = 0.9998）。回收率为90.2% - 93.8%，相对标准偏差为0.34%（n = 5）。该方法快速、简便、准确且可重现，可用于蜜桶花颗粒的质量控制。