[HPLC determination of acteoside in Radix Rehmanniae].

OBJECTIVE

To develop an HPLC method for the determination of acteoside in Radix Rehmanniae.

METHOD

The chromatographic conditions were as follows: Polaris C18(4.6 mm x 250 mm, 5 microm) column, a mobile phase in gradient mode composed of acetonitrile 0.1% acetic acid solution, a flow rate of 1.0 mL x min(-1), and 334 nm as the detection wavelength.

RESULT

Acteoside showed good linear relationship at the range of 10-500 microg x mL(-1) (r = 0.9990). The average recovery was 100.1%, RS D 3.7%.

CONCLUSION

The proposed method promised to be applicable for the quality control of Radix Rehmanniae.