Zhang Xiu-qiao, Huang Feng-jiao, Chen Jiao-chun, Liu Yan-wen
Hubei College of Traditional Chinese Medicine, Wuhan 430061, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1184-6.
To develop a RP-HPLC method for determination of three glycosides in Swertia punicea.
Chromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C.
The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5).
It is a simple and sensitive meathod in controlling the quality of S. punicea.
建立一种反相高效液相色谱法测定紫红獐牙菜中三种苷类成分。
色谱柱：Alltimal C18（4.6 mm×250 mm，5μm）。流动相：甲醇-水（含0.05% H3PO4），梯度洗脱。流速：1 mL·min-1。波长：254 nm。柱温：30℃。
龙胆苦苷、芒果苷和獐牙菜苦苷的校准曲线在31.3 - 281.7、0.31 - 2.78、0.55 - 4.91μg范围内线性关系良好，（r = 0.9996、0.9993、0.9995）。平均回收率分别为103.36%、101.42%和97.39%，相对标准偏差小于3%（n = 5）。
该方法简便、灵敏，可用于控制紫红獐牙菜的质量。
