Tashiro Ryu, Sugiyama Hiroshi
Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
J Am Chem Soc. 2005 Feb 23;127(7):2094-7. doi: 10.1021/ja044138j.
We demonstrate a molecular switch, on the basis of the characteristic properties of DNA and RNA, which indicates a completely inverted response to thermal stimuli using the transition between right- and left-handed helices. We designed a system using aminopurine (Ap), which can convert the pi-stack information of the transition from right-handed to left-handed DNA (B-Z transition) and RNA (A-Z transition) into an output giving a fluorescent signal. These two biomolecular devices together serve as "right-left" or "off-on" switches. When the temperature is changed from low to high, the RNA device changes from the off to on signal; however, the DNA device changes from on to off. The response of these RNA and DNA based devices against thermal stimulus was completely reversible.
我们展示了一种基于DNA和RNA特性的分子开关，该开关利用右手螺旋和左手螺旋之间的转变，对热刺激表现出完全相反的响应。我们设计了一个使用氨基嘌呤（Ap）的系统，它可以将右手DNA到左手DNA（B-Z转变）以及RNA（A-Z转变）转变过程中的π堆积信息转化为发出荧光信号的输出。这两个生物分子装置共同作为“右-左”或“关-开”开关。当温度从低变高时，RNA装置从关信号变为开信号；然而，DNA装置则从开信号变为关信号。这些基于RNA和DNA的装置对热刺激的响应是完全可逆的。
