使用 NMR 光谱研究具有非经典结构的 DNA 的动力学。
Dynamics Studies of DNA with Non-canonical Structure Using NMR Spectroscopy.
机构信息
Department of Chemistry and RINS, Gyeongsang National University, Gyeongnam 52828, Korea.
Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
出版信息
Int J Mol Sci. 2020 Apr 11;21(8):2673. doi: 10.3390/ijms21082673.
The non-canonical structures of nucleic acids are essential for their diverse functions during various biological processes. These non-canonical structures can undergo conformational exchange among multiple structural states. Data on their dynamics can illustrate conformational transitions that play important roles in folding, stability, and biological function. Here, we discuss several examples of the non-canonical structures of DNA focusing on their dynamic characterization by NMR spectroscopy: (1) G-quadruplex structures and their complexes with target proteins; (2) i-motif structures and their complexes with proteins; (3) triplex structures; (4) left-handed Z-DNAs and their complexes with various Z-DNA binding proteins. This review provides insight into how the dynamic features of non-canonical DNA structures contribute to essential biological processes.
非经典的核酸结构对于它们在各种生物过程中的多样化功能至关重要。这些非经典结构可以在多个结构状态之间进行构象交换。有关它们动力学的数据可以说明在折叠、稳定性和生物功能中起重要作用的构象转变。在这里，我们讨论了 DNA 的几种非经典结构的例子，重点讨论了通过 NMR 光谱学对其动态特性的表征：（1）G-四链体结构及其与靶蛋白的复合物；（2）i- 基序结构及其与蛋白质的复合物；（3）三螺旋结构；（4）左手 Z-DNAs 及其与各种 Z-DNA 结合蛋白的复合物。这篇综述提供了深入了解非经典 DNA 结构的动态特征如何有助于重要的生物过程。
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