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嗜热自养甲烷杆菌中的DNA含量及类核分布

DNA content and nucleoid distribution in Methanothermobacter thermautotrophicus.

作者信息

Majerník Alan I, Lundgren Magnus, McDermott Paul, Bernander Rolf, Chong James P J

机构信息

Department of Biology (Area 5), University of York, PO Box 373, York YO10 5YW, United Kingdom.

出版信息

J Bacteriol. 2005 Mar;187(5):1856-8. doi: 10.1128/JB.187.5.1856-1858.2005.

Abstract

Flow cytometry and epifluorescence microscopy results for the euryarchaeon Methanothermobacter thermautotrophicus were consistent with filaments containing multiple cells. Filaments of one to four cells contained two to eight nucleoids. Single chromosome-containing cells were not observed. Filaments containing multiple genome copies displayed synchronous DNA replication initiation. Chromosome segregation occurred during replication or rapidly after replication termination.

摘要

嗜热自养甲烷杆菌的流式细胞术和落射荧光显微镜结果与包含多个细胞的丝状体一致。一到四个细胞的丝状体含有两到八个类核。未观察到含有单个染色体的细胞。包含多个基因组拷贝的丝状体显示出同步的DNA复制起始。染色体分离发生在复制期间或复制终止后迅速发生。

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