Jahan Nayyar, Shahid Faiza, Aman Afsheen, Mujahid Talat Yasmeen, Qader Shah Ali Ul
Department of Microbiology, University of Karachi, Karachi-75270, Pakistan.
The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi-75270, Pakistan.
Heliyon. 2017 Jun 20;3(6):e00330. doi: 10.1016/j.heliyon.2017.e00330. eCollection 2017 Jun.
In the present study, a variety of agro-industrial wastes have been utilized for meaningful purpose to produce valuable biocatalyst. All wastes used were low cost and easily accessible while, some available throughout the year. A number of bacterial strains isolated from rotten fruits and vegetables were screened for the production of industrially important polygalacturonase (PGase) using pectin present in these agro-industrial wastes. The strain producing maximum PGase was identified as KIBE-IB3 on the basis of taxonomic studies and 16S rDNA analysis. Among different agro-industrial wastes studied, high yield of PGase was achieved from fermentation broth having wheat bran (1.0%) as a substrate in to the medium supplemented with nitrogen sources in combination of NaNO and yeast extract while KHPO was selected as suitable micronutrient. After optimizing fermentation parameters it was noticed that KIBE-IB3 was capable of producing maximum PGase at 37 °C, pH 7.0 and after 48 h of incubation time. From the current research, wheat bran was proven as a cheap and easily available source throughout the year for hyper production of pectinase. The utilization of the waste will also help to minimize the concerned environmental issues.
在本研究中，多种农业工业废弃物已被用于有意义的目的，以生产有价值的生物催化剂。所有使用的废弃物成本低廉且易于获取，有些全年都有。从腐烂的水果和蔬菜中分离出许多细菌菌株，利用这些农业工业废弃物中存在的果胶筛选产工业上重要的聚半乳糖醛酸酶（PGase）的菌株。根据分类学研究和16S rDNA分析，产PGase量最高的菌株被鉴定为KIBE-IB3。在所研究的不同农业工业废弃物中，以含有1.0%麦麸作为底物的发酵液，在添加NaNO和酵母提取物作为氮源组合且选择KHPO作为合适微量营养素的培养基中，可实现PGase的高产。优化发酵参数后发现，KIBE-IB3在37℃、pH 7.0且培养48小时后能够产生最大量的PGase。从当前研究来看，麦麸被证明是全年用于超量生产果胶酶的廉价且易于获取的来源。废弃物的利用也将有助于最大限度地减少相关环境问题。
