Kon Tatsuya, Tanigawa Toru, Hayamizu Kohsuke, Shen Manzhen, Tsuji Tomoko, Naito Yuji, Yoshikawa Toshikazu
Central Research Laboratory, FANCL Corporation, Kanagawa, Yokohama, Japan.
Redox Rep. 2004;9(6):325-30. doi: 10.1179/135100004225006821.
Singlet oxygen is regarded as contributing to the pathogenesis of various diseases including light-induced skin disorders and inflammatory response. In this study, the correlation between singlet oxygen quenching activity (SOQA) of human serum and blood biochemistry or life-style was evaluated. Healthy volunteers were recruited and carried out a measurement of SOQA by using electron paramagnetic resonance (EPR) and a questionnaire survey about a smoking. It was demonstrated that major quenchers of singlet oxygen in serum are proteins, and small molecular anti-oxidants relatively play a minor role. SOQA of whole sera showed no correlation with protein concentration, but positively correlated with SOQA of small molecular fraction. In vitro studies demonstrated that the decrease of sulfhydryl groups by NO or superoxide significantly attenuated SOQA of albumin. Together, these results may imply that the underlying oxidative condition in each individual influences both small molecular antioxidant states and the sulfhydryl content of serum proteins. SOQA of sera from women with a smoking history was significantly lower compared to non-smoking women, suggesting that the smoking habit impaired the defense mechanism against singlet oxygen.
单线态氧被认为与包括光诱导的皮肤疾病和炎症反应在内的各种疾病的发病机制有关。在本研究中，评估了人血清的单线态氧猝灭活性（SOQA）与血液生化或生活方式之间的相关性。招募了健康志愿者，通过电子顺磁共振（EPR）测量SOQA，并进行了关于吸烟的问卷调查。结果表明，血清中单线态氧的主要猝灭剂是蛋白质，小分子抗氧化剂相对起次要作用。全血清的SOQA与蛋白质浓度无相关性，但与小分子部分的SOQA呈正相关。体外研究表明，NO或超氧化物使巯基减少会显著减弱白蛋白的SOQA。综上所述，这些结果可能意味着个体潜在的氧化状态会影响小分子抗氧化剂状态和血清蛋白的巯基含量。有吸烟史女性的血清SOQA明显低于非吸烟女性，这表明吸烟习惯损害了对抗单线态氧的防御机制。
