Avinash S S, Anitha M, Rao Gayathri M, Sudha K, Shetty Beena V
Department of Biochemistry, Centre for Basic Sciences, Kasturba Medical College, Bejai, Mangalore - 575 004.
Indian J Physiol Pharmacol. 2009 Oct-Dec;53(4):370-4.
The present study was designed to assess the levels of advanced oxidation protein products (AOPP) and percent hemolysis (that indirectly indicates the degree of membrane damage secondary to lipid peroxidation) in colorectal carcinoma. Glutathione (GSH), total thiols and albumin were measured to determine the antioxidant status. Considering the dynamic interaction between various antioxidants in the body, we measured the total antioxidant activity (AOA). Globulin was measured to assess the inflammatory response secondary to oxidative stress. Investigations were conducted in 45 cases of recently diagnosed primary colorectal adenocarcinoma. As control, 45 age and sex matched healthy persons were chosen. GSH was estimated in whole blood, percent hemolysis in RBC suspension and other parameters in plasma. We observed a very high significant increase (P<0.001) in AOPP, percent hemolysis and a highly significant increase (P<0.01) in globulin in colorectal carcinoma. We observed a very high significant decrease (P<0.001) in whole blood GSH, total thiols, albumin, AOA and a significant decrease (P<0.05) in plasma GSH in colorectal carcinoma. A very high significant negative correlation between percent hemolysis and AOA and an apparent negative correlation between total thiols and AOPP was seen in colorectal carcinoma. This demonstrated oxidative stress, decreased antioxidant status and secondary inflammatory response in colorectal carcinoma.
本研究旨在评估结直肠癌患者体内晚期氧化蛋白产物（AOPP）水平以及溶血百分比（间接反映脂质过氧化继发的膜损伤程度）。检测谷胱甘肽（GSH）、总硫醇和白蛋白以确定抗氧化状态。考虑到体内各种抗氧化剂之间的动态相互作用，我们检测了总抗氧化活性（AOA）。检测球蛋白以评估氧化应激继发的炎症反应。对45例新诊断的原发性结直肠癌患者进行了研究。作为对照，选取了45名年龄和性别匹配的健康人。检测全血中的GSH、红细胞悬液中的溶血百分比以及血浆中的其他参数。我们观察到，结直肠癌患者的AOPP、溶血百分比显著升高（P<0.001），球蛋白显著升高（P<0.01）。我们还观察到，结直肠癌患者全血中的GSH、总硫醇、白蛋白、AOA显著降低（P<0.001），血浆中的GSH显著降低（P<0.05）。在结直肠癌患者中，溶血百分比与AOA之间存在极显著的负相关，总硫醇与AOPP之间存在明显的负相关。这表明结直肠癌患者存在氧化应激、抗氧化状态降低以及继发的炎症反应。
