Aslan Kadir, Gryczynski Ignacy, Malicka Joanna, Matveeva Evgenia, Lakowicz Joseph R, Geddes Chris D
Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA.
Curr Opin Biotechnol. 2005 Feb;16(1):55-62. doi: 10.1016/j.copbio.2005.01.001.
Over the past 15 years, fluorescence has become the dominant detection/sensing technology in medical diagnostics and biotechnology. Although fluorescence is a highly sensitive technique, where single molecules can readily be detected, there is still a drive for reduced detection limits. The detection of a fluorophore is usually limited by its quantum yield, autofluorescence of the samples and/or the photostability of the fluorophores; however, there has been a recent explosion in the use of metallic nanostructures to favorably modify the spectral properties of fluorophores and to alleviate some of these fluorophore photophysical constraints. The use of fluorophore-metal interactions has been termed radiative decay engineering, metal-enhanced fluorescence or surface-enhanced fluorescence.
在过去的15年里，荧光已成为医学诊断和生物技术领域占主导地位的检测/传感技术。尽管荧光是一种高度灵敏的技术，单分子很容易被检测到，但降低检测限的需求仍然存在。荧光团的检测通常受其量子产率、样品的自发荧光和/或荧光团的光稳定性限制；然而，最近金属纳米结构的应用激增，可有利地改变荧光团的光谱特性，并缓解其中一些荧光团的光物理限制。荧光团与金属相互作用的应用被称为辐射衰变工程、金属增强荧光或表面增强荧光。
