Liu Yaoming, Sun Shan, Singha Sima, Cho Michael R, Gordon Robert J
Department of Chemistry (m/c 111), University of Illinois at Chicago, 845 W Taylor Street, Chicago, IL 60607, USA.
Biomaterials. 2005 Aug;26(22):4597-605. doi: 10.1016/j.biomaterials.2004.11.033.
Three-dimensional micropatterned collagen scaffolds were fabricated by femtosecond laser ablation. An 800 nm, 45 fs Ti:Sapphire laser was used to create various 3D patterns in a collagen gel, including holes, lines and grids. An optimal collagen concentration was found for both substrate patterning and cell compatibility. The threshold fluence for ablation of the scaffold was found to be 0.0 6 J/cm(2), and the morphology of the ablation craters was measured as a function of fluence. Mesenchymal stem cells from rat bone marrow and human fibroblasts were seeded within the ablated patterns and were shown to be viable for at least 10 days.
通过飞秒激光烧蚀制备了三维微图案化胶原蛋白支架。使用波长800 nm、脉宽45 fs的钛宝石激光在胶原蛋白凝胶中创建各种三维图案，包括孔、线和网格。发现了用于底物图案化和细胞相容性的最佳胶原蛋白浓度。发现支架烧蚀的阈值通量为0.06 J/cm²，并测量了烧蚀坑的形态随通量的变化。将大鼠骨髓间充质干细胞和人成纤维细胞接种在烧蚀图案内，结果显示它们至少存活了10天。
