Mohammed Khairallah A S, Miles Roger J, Halablab Mahmoud A
Department of Life Sciences, Franklin-Wilkins Building, King's College London, 150 Stamford Street, London SE1 9NN, UK.
J Microbiol Methods. 2005 May;61(2):273-6. doi: 10.1016/j.mimet.2004.11.014. Epub 2004 Dec 24.
Campylobacter strains (100 human, animal and environmental isolates) were grown in untreated brain heart infusion broth medium (10 ml in tightly capped 20 ml capacity universal tubes) without using microaerophilic kits. Cells grown in these conditions did not differ in their growth rates, protein profiles or substrate utilisation even after 40 passages compared to cells grown under microaerophilic conditions. Growth in such conditions provides a cost effective, convenient and simple system for growing pure culture of campylobacters and obviates the generation of microaerobic conditions using specialised kits.
弯曲杆菌菌株（100株人源、动物源和环境分离株）在未处理的脑心浸液肉汤培养基（10毫升，置于容量为20毫升的密封通用管中）中培养，未使用微需氧试剂盒。在这些条件下生长的细胞，与在微需氧条件下生长的细胞相比，即使经过40代传代，其生长速率、蛋白质谱或底物利用情况也没有差异。在这种条件下生长为弯曲杆菌纯培养物的培养提供了一种经济高效、方便且简单的系统，无需使用专门的试剂盒来产生微需氧条件。
