Laine Pia, Savolainen Carita, Blomqvist Soile, Hovi Tapani
Department of Microbiology, Enterovirus Laboratory, National Public Health Institute (KTL), Mannerheimintie 166, 00300 Helsinki, Finland.
J Gen Virol. 2005 Mar;86(Pt 3):697-706. doi: 10.1099/vir.0.80445-0.
Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human rhinovirus (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the established two species, Human rhinovirus A (HRV-A) and Human rhinovirus B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % between the two HRV species. Interserotypic sequence identities in both HRV species were more variable than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification by VP1 sequence analysis used for HEV strains may not always be possible for HRV isolates. On the other hand, a comprehensive insight into the relationships between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. Distribution of pairwise nucleotide identity values between these genera varied from 41 to 54 % in the VP1 coding region, similar to those between heterologous members of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues between HRV-B and polioviruses than between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together but behaved like members of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.
对所有101株人鼻病毒(HRV)原型株的衣壳蛋白VP1编码序列进行系统发育分析,发现了两个主要的基因簇,这与先前报道的VP4/VP2编码序列的情况相似,代表了已确定的两个种,即人鼻病毒A(HRV-A)和人鼻病毒B(HRV-B)。两个HRV种内的成对核苷酸同一性在61%至98%之间,而种间则在46%至55%之间。两种HRV种内的血清型间序列同一性比同一科内任何人类肠道病毒(HEV)种内的序列同一性变化更大。这意味着,用于HEV毒株的通过VP1序列分析进行明确的血清型鉴定,对于HRV分离株可能并不总是可行的。另一方面,对HRV和HEV的VP1与部分2A序列之间关系的全面洞察揭示了一种类似属的情况。这些属之间在VP1编码区的成对核苷酸同一性值分布在41%至54%之间,与两种HRV种的异源成员之间的情况相似。推导的氨基酸序列比对显示,HRV-B与脊髓灰质炎病毒之间比两种HRV种之间有更多完全保守的氨基酸残基。在包含所有HRV和所有HEV种代表的系统发育树中,两种HRV种并未聚在一起,而是表现得像与HEV属于同一属的成员。总之,从系统发育的角度来看,没有充分的理由在分类学上保持这两个人微小核糖核酸病毒属的分离。