Peters Brock A, Diaz Luis A, Polyak Kornelia, Meszler Leslie, Romans Kathy, Guinan Eva C, Antin Joseph H, Myerson David, Hamilton Stanley R, Vogelstein Bert, Kinzler Kenneth W, Lengauer Christoph
The Sidney Kimmel Comprehensive Cancer Center and Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, Maryland 21231, USA.
Nat Med. 2005 Mar;11(3):261-2. doi: 10.1038/nm1200. Epub 2005 Feb 20.
It has been shown that bone marrow-derived stem cells can form a major fraction of the tumor endothelium in mouse tumors. To determine the role of such cells in human tumor angiogenesis, we studied six individuals who developed cancers after bone marrow transplantation with donor cells derived from individuals of the opposite sex. By performing fluorescence in situ hybridization (FISH) with sex chromosome-specific probes in conjunction with fluorescent antibody staining, we found that such stem cells indeed contributed to tumor endothelium, but at low levels, averaging only 4.9% of the total. These results illustrate substantial differences between human tumors and many mouse models with respect to angiogenesis and have important implications for the translation of experimental antiangiogenic therapies to the clinic.
研究表明，骨髓来源的干细胞可在小鼠肿瘤中构成肿瘤内皮的主要部分。为了确定此类细胞在人类肿瘤血管生成中的作用，我们研究了6名在接受异性个体来源的供体细胞进行骨髓移植后发生癌症的患者。通过使用性染色体特异性探针进行荧光原位杂交（FISH）并结合荧光抗体染色，我们发现此类干细胞确实对肿瘤内皮有贡献，但水平较低，平均仅占总数的4.9%。这些结果表明，人类肿瘤与许多小鼠模型在血管生成方面存在显著差异，并且对于将实验性抗血管生成疗法转化至临床具有重要意义。
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