[高效液相色谱法测定雷公藤总萜片中毒素含量]
[Determination of triptolide in Tripterygium total terpenoids tablets by HPLC].
作者信息
Pu Cun-hai, He Ming-fang, Zhanag Xiao-qian, Shen Jia
机构信息
Nanjing Zhongshan Pharmaceutical Factory, Nanjing 210012, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 Feb;30(4):266-7.
OBJECTIVE
To develop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets.
METHOD
A Lichrospher CN column was used with ethanol and water for gradient elution. The detection wavelength was set at 255 nm.
RESULT
The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 microg x mL(-1) (r = 0.9998). The average recovery was 99.2%, RSD% = 1.7%.
CONCLUSION
The method is simple, rapid and accurate and can be used for quality control of the tablets.
目的
建立一种测定雷公藤总萜片中毒素内酯含量的高效液相色谱法。
方法
采用Lichrospher CN柱，以乙醇和水进行梯度洗脱。检测波长设定为255nm。
结果
在0.742 - 59.4μg·mL⁻¹范围内，浓度与峰面积线性关系良好（r = 0.9998）。平均回收率为99.2%，相对标准偏差（RSD%）为1.7%。
结论
该方法简便、快速、准确，可用于该片剂的质量控制。
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