[薄层扫描法测定中药雷公藤中雷公藤酚内酯的含量]
[Determination of triptophenolide in Chinese herb leigongteng by TLCS].
作者信息
Wang X, Zhou L
机构信息
Guangzhou University of TCM, Guangzhou 510407.
出版信息
Zhongguo Zhong Yao Za Zhi. 1999 Jan;24(1):14-5, 62.
PMID:12078147
Abstract
OBJECTIVE
To establish a method for the determination of triptophenolide in Chinese herb Leigongteng (Tripterygium wilfordii Hook. f.).
METHOD
TLCS was used. The sample was purified with a column and developed upwardly with CHCl3-EtOEt(2:1), sprayed with Keddle testing solution and scanned. The content was calculated with external two-point method.
RESULT
The linear range was 2.1 micrograms-10.5 micrograms, r = 0.998.
CONCLUSION
The method is proper for the quality control of Leigongteng.
摘要
目的
建立中药雷公藤（Tripterygium wilfordii Hook. f.）中雷公藤酚内酯的含量测定方法。
方法
采用薄层扫描法。样品经柱色谱纯化后，以三氯甲烷-乙酸乙酯（2∶1）为展开剂上行展开，喷以 Kedde 试液，进行扫描。采用外标两点法计算含量。
结果
线性范围为 2.1μg～10.5μg，r = 0.998。
结论
该方法适用于雷公藤的质量控制。
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