Tripathy Srikanth P, Kulkarni Smita S, Jadhav Sushama D, Agnihotri Kalpana D, Jere Abhay J, Kurle Swarali N, Bhattacharya Sujit K, Singh Khomdon, Tripathy Sriram P, Paranjape Ramesh S
National AIDS Research Institute, 73 G Block, MIDC, Bhosari, Pune-411026, India.
AIDS Res Hum Retroviruses. 2005 Feb;21(2):152-7. doi: 10.1089/aid.2005.21.152.
The predominant HIV-1 strain circulating in India is subtype C. However, subtype A and B strains of HIV-1 have also been reported in India. In 1999, the first A/C recombinant strain was reported from Pune in India. Intravenous drug users (IVDUs) from the northeastern region of India have a high HIV-1 seroprevalence. Studies carried out in intravenous drug users in the northeastern region of India have shown that HIV-1 subtype C is the predominant strain infecting IVDUs. Fourteen blood samples were collected from HIV-1-infected individuals from the northeastern region of India and screened by env and gag heteroduplex mobility assays (HMA). Where the env and gag HMA results from a sample yielded different subtypes, sequencing of env and gag PCR products was carried out to confirm the presence of HIV-1 recombinants. Of the 14 samples subtyped, nine samples belonged HIV-1 subtype C (gag C/env C), one to HIV-1 subtype B (gag B/env B), and the remaining were B/C recombinants (gag C/env B). This is the first report of HIV-1 B/C recombinants from India.
在印度流行的主要HIV-1毒株是C亚型。然而，HIV-1的A和B亚型毒株在印度也有报道。1999年，印度浦那报道了首例A/C重组毒株。印度东北地区的静脉吸毒者中HIV-1血清阳性率很高。在印度东北地区对静脉吸毒者开展的研究表明，HIV-1 C亚型是感染静脉吸毒者的主要毒株。从印度东北地区的HIV-1感染者中采集了14份血样，并通过env和gag异源双链迁移率分析（HMA）进行筛查。如果某个样本的env和gag HMA结果显示为不同亚型，则对env和gag PCR产物进行测序，以确认HIV-1重组体的存在。在14份分型的样本中，9份样本属于HIV-1 C亚型（gag C/env C），1份属于HIV-1 B亚型（gag B/env B），其余为B/C重组体（gag C/env B）。这是印度关于HIV-1 B/C重组体的首次报道。
