Pu Lee L Q, Cui Xiangdong, Fink Betsy F, Cibull Michael L, Gao Dayong
Division of Plastic Surgery, University of Kentucky, Lexington, KY 40536-0284, USA.
Ann Plast Surg. 2005 Mar;54(3):288-92; discussion 292.
This study was conducted to evaluate the viability of fatty tissues within adipose aspirates after conventional liposuction and to examine their potential role as a source of donor material for possible future autogenous fat grafting. Samples of adipose aspirates (group I, n = 8) were obtained from adult female patients who underwent a conventional liposuction of the abdomen. Samples of fresh fatty tissues obtained from adult female patients who underwent an abdominoplasty (group II, n = 8) were cut into small pieces and served as a control. All samples were spun at 50 x g for 10 minutes; fatty tissues were then collected from the middle layer after centrifugation for the following studies: trypan blue vital staining for viable fatty cell counts, glycerol-3-phophatase dehydrogenase (G3PDH) assay for functional evaluation of fatty tissues, and routine pathology for histology of fatty tissues. There was no significant difference of viable fatty cell counts in group I compared with group II (2.57 +/- 0.56 versus 2.74 +/- 0.59 x 10/mL, P = 0.56). G3PGH assay showed a marked decrease of the enzyme activity in group I compared with group II (0.34 +/- 0.13 versus 0.76 +/- 0.13 micro/mL, P < 0.0001). Histologically, the normal structure of fatty tissues was found primarily in both groups. Our results indicate that although fatty tissues within adipose aspirates after conventional liposuction maintain normal structure with near the same number of viable fatty cells compared with fresh ones, they have a less-than-optimal level of cellular function and may not survive well after they are transplanted.
本研究旨在评估传统抽脂术后脂肪抽吸物中脂肪组织的活力，并探讨其作为未来自体脂肪移植供体材料来源的潜在作用。脂肪抽吸物样本（第一组，n = 8）取自接受腹部传统抽脂术的成年女性患者。将接受腹部整形术的成年女性患者（第二组，n = 8）获取的新鲜脂肪组织切成小块作为对照。所有样本以50×g离心10分钟；然后从离心后的中间层收集脂肪组织用于以下研究：用台盼蓝活体染色法计数存活脂肪细胞，用甘油-3-磷酸脱氢酶（G3PDH）测定法评估脂肪组织的功能，以及用常规病理学方法观察脂肪组织的组织学情况。与第二组相比，第一组存活脂肪细胞计数无显著差异（2.57±0.56对2.74±0.59×10/mL，P = 0.56）。G3PGH测定显示，与第二组相比，第一组酶活性显著降低（0.34±0.13对0.76±0.13微升/毫升，P < 0.0001）。组织学上，两组主要均发现脂肪组织结构正常。我们的结果表明，尽管传统抽脂术后脂肪抽吸物中的脂肪组织与新鲜脂肪组织相比保持正常结构且存活脂肪细胞数量相近，但其细胞功能水平欠佳，移植后可能无法良好存活。
