Department of Plastic and Reconstructive Surgery, Soonchunhyang University Hospital, Gumi 39371, Korea.
Department of Integrated Biomedical Science, Soonchunhyang Institute of Medi-Bio Science (SIMS), Soonchunhyang University, Cheonan 31151, Korea.
Int J Mol Sci. 2022 Oct 6;23(19):11839. doi: 10.3390/ijms231911839.
Cell-assisted lipotransfer (CAL), defined as co-transplantation of aspirated fat with enrichment of adipose-derived stem cells (ASCs), is a novel technique for cosmetic and reconstructive surgery to overcome the low survival rate of traditional fat grafting. However, clinically approved techniques for increasing the potency of ASCs in CAL have not been developed yet. As a more clinically applicable method, we used mechanical stress to reinforce the potency of ASCs. Mechanical stress was applied to the inguinal fat pad by needling . Morphological and cellular changes in adipose tissues were examined by flow cytometric analysis 1, 3, 5, and 7 days after the procedure. The proliferation and adipogenesis potencies of ASCs were evaluated. CAL with ASCs treated with mechanical stress or sham control were performed, and engraftment was determined at 4 weeks post-operation. Flow cytometry analysis revealed that mechanical stress significantly increased the number as well as the frequency of ASC proliferation in fat. Proliferation assays and adipocyte-specific marker gene analysis revealed that mechanical stress promoted proliferation potential but did not affect the differentiation capacity of ASCs. Moreover, CAL with cells derived from mechanical stress-treated fat increased the engraftment. Our results indicate that mechanical stress may be a simple method for improving the efficacy of CAL by enhancing the proliferation potency of ASCs.
细胞辅助脂肪转移（CAL），定义为抽吸脂肪与脂肪来源干细胞（ASCs）的富集共移植，是一种用于美容和重建手术的新技术，旨在克服传统脂肪移植的低存活率。然而，目前尚未开发出临床上可提高 CAL 中 ASC 效力的技术。作为一种更具临床应用前景的方法，我们使用机械应力来增强 ASC 的效力。通过针刺对腹股沟脂肪垫施加机械应力。在手术后 1、3、5 和 7 天，通过流式细胞术分析检查脂肪组织的形态和细胞变化。评估 ASC 的增殖和脂肪生成能力。进行了经机械应力处理或假对照处理的 ASC 的 CAL，并在术后 4 周时确定了植入情况。流式细胞术分析显示，机械应力显著增加了脂肪中 ASC 的增殖数量和频率。增殖测定和脂肪细胞特异性标记基因分析表明，机械应力促进了增殖潜能，但不影响 ASC 的分化能力。此外，源自经机械应力处理的脂肪的细胞的 CAL 增加了植入。我们的结果表明，机械应力可能是一种通过增强 ASC 的增殖能力来提高 CAL 效果的简单方法。
