Szepetowski P, Simon M P, Grosgeorge J, Huebner K, Bastard C, Evans G A, Tsujimoto Y, Birnbaum D, Theillet C, Gaudray P
LGMCH, CNRS URA 1462, Nice, France.
Genomics. 1992 Apr;12(4):738-44. doi: 10.1016/0888-7543(92)90303-a.
We have employed two strategies to map 13 markers located at 11q13. First, we used pulsed-field gel electrophoresis of DNA fragments obtained with methylation-sensitive restriction enzymes. The markers used in this study were scattered over 8.4 Mb and, for most of them, could not be linked one to another. A second mapping strategy employed hybridization to either DNA of somatic hybrids containing various parts of the long arm of chromosome 11 or metaphase chromosomes of a B-cell line containing the t(11;14)(q13;q32) translocation. We were able to sort out the centromeric from the telomeric probes with respect to translocation breakpoints taken as reference chromosomal landmarks by this approach. BCL1, which corresponds to the region where the t(11;14)(q13;q32) translocation breakpoints are clustered, appears as a boundary between two areas of human/mouse homology present in conserved syntenic regions on mouse chromosomes 7 and 19.
我们采用了两种策略来定位位于11q13的13个标记。首先，我们对用甲基化敏感限制性内切酶获得的DNA片段进行脉冲场凝胶电泳。本研究中使用的标记分布在8.4 Mb范围内，并且对于大多数标记而言，无法将它们彼此相连。第二种定位策略是与包含11号染色体长臂不同部分的体细胞杂种DNA或含有t(11;14)(q13;q32)易位的B细胞系的中期染色体进行杂交。通过这种方法，相对于作为参考染色体界标的易位断点，我们能够区分着丝粒探针和端粒探针。BCL1对应于t(11;14)(q13;q32)易位断点聚集的区域，它是小鼠7号和19号染色体上保守同线性区域中存在的两个人/小鼠同源性区域之间的边界。
