Zhang Yu, Chen Shao-Hua, Zhang Xing-Guo, Ren Guo-Ping, Sa Xiao-Ying, Yu Chao-Hui, Li You-Ming
Department of Gastroenterology, the First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
Zhonghua Gan Zang Bing Za Zhi. 2005 Feb;13(2):125-7.
To reproduce an experimental model of alcoholic liver disease in rats and to investigate the preventive and treatment effects of tea polyphenols on alcoholic liver disease.
68 male Sprague-Dawley rats were randomly divided into 3 groups: alcohol group (gastrically infused with 56% of ethanol once a day with a dose of 7 g/kg body weight for 4, 12 and 24 weeks), tea polyphenols group (gastric infusion with alcohol same as in the alcohol group and with tea polyphenols at 0.25 g/kg bw) and control group (gastric infusion with normal saline). At the end of 4, 12 and 24 weeks, blood samples were collected and then the rats were sacrificed. Liver samples were obtained for routine histological examination and the degree of hepatic steatosis and alcoholic hepatitis were examined. Blood specimens were used for evaluation of alanine transaminase (ALT) and aspartate aminotransferase (AST).
(1) The levels of the two transaminases were elevated with the increase of the duration of ethanol feeding and the difference is significant. TP significantly mitigated the increase of ALT and AST activities induced by the alcohol. (2) Histological changes of the liver injury indicated that piecemeal or focal necrosis of hepatocytes was present in the centrilobular area. As fibrosis advanced, broader septa were formed with central-central and centra-portal bridging necrosis. In the TP infusion group, the severity of the pathological changes was significantly milder.
The results of this study revealed that TP mitigated the development of alcoholic liver disease, and TP may be a potential drug for treatment of alcoholic liver disease.
复制大鼠酒精性肝病实验模型，探讨茶多酚对酒精性肝病的防治作用。
将68只雄性Sprague-Dawley大鼠随机分为3组：酒精组（每天灌胃56%乙醇，剂量为7 g/kg体重，分别持续4、12和24周）、茶多酚组（灌胃酒精同酒精组，同时灌胃茶多酚0.25 g/kg体重）和对照组（灌胃生理盐水）。在4、12和24周结束时，采集血样，然后处死大鼠。获取肝脏样本进行常规组织学检查，观察肝脂肪变性程度和酒精性肝炎情况。血标本用于评估丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）。
（1）随着乙醇喂养时间延长，两种转氨酶水平升高，差异有统计学意义。茶多酚显著减轻了酒精诱导的ALT和AST活性升高。（2）肝损伤的组织学变化表明，小叶中央区存在肝细胞碎片状或灶性坏死。随着纤维化进展，形成更宽的间隔，伴有中央-中央和中央-门静脉桥接坏死。在茶多酚灌胃组，病理变化的严重程度明显较轻。
本研究结果表明，茶多酚减轻了酒精性肝病的发展，茶多酚可能是治疗酒精性肝病的一种潜在药物。
