Silva Francinaldo, Gomes Regis, Prates Deboraci, Miranda José C, Andrade Bruno, Barral-Netto Manoel, Barral Aldina
Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Salvador, Bahia, Brazil.
Am J Trop Med Hyg. 2005 Jan;72(1):94-8.
Sand flies inject saliva into the mammalian host when probing for a blood meal. Understanding the initial vertebrate reactions against sand fly saliva is important for possible interventions because these insects transmit diseases to humans and other animals. Little is known of these reactions to New World sand flies. Repeated exposure of BALB/c mice to Lutzomyia longipalpis bites leads to local inflammatory cell infiltration comprised of neutrophils, macrophages, and eosinophils. Total IgG and IgG1 antibodies react predominantly with three major protein bands (45, 44, and 16 kD) of the insect saliva by Western blot. The injection of immune serum previously incubated with salivary gland homogenate induced an early infiltration with neutrophils and macrophages, suggesting the participation of immune complexes in triggering inflammation.
白蛉在探寻血餐时会将唾液注入哺乳动物宿主。了解脊椎动物对白蛉唾液的初始反应对于可能的干预措施很重要，因为这些昆虫会将疾病传播给人类和其他动物。对于这些对新大陆白蛉的反应知之甚少。将BALB/c小鼠反复暴露于长须白蛉叮咬下会导致局部炎症细胞浸润，包括中性粒细胞、巨噬细胞和嗜酸性粒细胞。通过蛋白质印迹法，总IgG和IgG1抗体主要与昆虫唾液的三条主要蛋白带（45、44和16 kD）发生反应。注射先前与唾液腺匀浆孵育的免疫血清会诱导中性粒细胞和巨噬细胞早期浸润，这表明免疫复合物参与引发炎症。
