Ledón N, Romay Ch, Rodríguez V, Cruz J, Rodríguez S, Ancheta O, González A, González R, Tolón Z, Cano M, Rojas E, Capote A, Valdes T
Molecular Immunology Center, National Center for Scientific Research, Havana Cuba.
Planta Med. 2005 Feb;71(2):126-9. doi: 10.1055/s-2005-837778.
A mixture of fatty acids obtained from sugar cane wax oil, the main components of which are palmitic, oleic, linoleic and linolenic acids, was evaluated topically in two experimental models of hypersensitivity: the ear swelling response to ovalbumin in sensitized mice (ED50 edema: 0.63 +/- 0.06 mg/ear, ED50 myeloperoxidase: 0.56 +/- 0.04 mg/ear, ED50 degranulated cells: 0.70 +/- 0,08 mg/ear) and oxazolone-induced contact hypersensitivity in mice (ED50 edema: 1.63 +/- 0.26 mg/ear, ED50 myeloperoxidase: 1.50 +/- 0.28 mg/ear, ED50 degranulated cells: 1.69 +/- 0.08 mg/ear). Also, the effect of this mixture was studied on the chemotaxis induced by fmlp (ED50: 25 +/- 3 microg/mL). The mixture showed anti-inflammatory activity in both in vivo models of allergy and in the chemotaxis test. Therefore, these results provide evidence about the potential usefulness of the mixture of fatty acids from sugar cane wax oil in cutaneous inflammatory and allergic disorders.
对从甘蔗蜡油中提取的脂肪酸混合物进行了评估，其主要成分是棕榈酸、油酸、亚油酸和亚麻酸，采用两种超敏反应实验模型进行局部给药：致敏小鼠对卵清蛋白的耳部肿胀反应（半数有效量[ED50]水肿：0.63±0.06毫克/耳，ED50髓过氧化物酶：0.56±0.04毫克/耳，ED50脱颗粒细胞：0.70±0.08毫克/耳）以及小鼠恶唑酮诱导的接触性超敏反应（ED50水肿：1.63±0.26毫克/耳，ED50髓过氧化物酶：1.50±0.28毫克/耳，ED50脱颗粒细胞：1.69±0.08毫克/耳）。此外，还研究了该混合物对甲酰甲硫氨酸-亮氨酸-苯丙氨酸（fmlp）诱导的趋化作用的影响（ED50：25±3微克/毫升）。该混合物在两种体内过敏模型以及趋化试验中均表现出抗炎活性。因此，这些结果为甘蔗蜡油脂肪酸混合物在皮肤炎症和过敏性疾病中的潜在用途提供了证据。
