Weeks Jason M, Spurgeon David J, Svendsen Claus, Hankard Peter K, Kammenga Jan E, Dallinger Reinhard, Köhler Heinz-R, Simonsen Vibeke, Scott-Fordsmand Janeck
Centre for Environment, Fisheries and Aquaculture Sciences (CEFAS), Pakefield Road, Lowestoft, Suffolk NR33 OHT, United Kingdom.
Ecotoxicology. 2004 Nov;13(8):817-22. doi: 10.1007/s10646-003-4479-z.
During the period 1996-1999 a joint field research programme (BIOPRINT-II) funded by the European Union was undertaken. The main objective of this project was the deployment of biochemical fingerprint techniques of soil invertebrate biomarkers for assessing the exposure and effect of toxicants on soil invertebrates in the field. The aim was to apply these techniques in the field focusing on a a chronically polluted field near a lead and zinc smelter in Avonmouth (UK). Therefore six sites were selected from which organisms were either sampled or transplanted to or from the laboratory. The project has provided a unique opportunity to apply a series of biological test methodologies in order to determine the hazard posed to soil sustainability and by inference soil biodiversity and function. This work has attempted to understand the linkage between effects measured at the molecular or cellular level and relate these to changes at higher levels of biological organisation. Here we evaluated the links between biomarkers and soil function parameters. The paper aims to summarize and explore the necessary caveats that must be understood before soil biomarker test systems may be used to strengthen the risk assessment process.
在1996年至1999年期间，开展了一项由欧盟资助的联合实地研究项目（BIOPRINT-II）。该项目的主要目标是部署土壤无脊椎动物生物标志物的生化指纹技术，以评估野外有毒物质对土壤无脊椎动物的暴露和影响。目的是在英国埃文茅斯一家铅锌冶炼厂附近的一个长期受污染的场地应用这些技术。因此，从六个地点采集了生物样本，或者将生物样本从野外采集到实验室，或者从实验室移植到野外。该项目提供了一个独特的机会，应用一系列生物学测试方法来确定对土壤可持续性以及由此推断的土壤生物多样性和功能所构成的危害。这项工作试图了解在分子或细胞水平上测得的效应之间的联系，并将这些联系与更高生物组织水平上的变化联系起来。在此，我们评估了生物标志物与土壤功能参数之间的联系。本文旨在总结和探讨在使用土壤生物标志物测试系统加强风险评估过程之前必须了解的必要注意事项。
