Morato E P, Leomil L, Beutin L, Krause G, Moura R A, Pestana de Castro A F
Departamento de Microbiologia, Universidade de São Paulo, SP, Brazil.
Zoonoses Public Health. 2009 Jun;56(5):229-37. doi: 10.1111/j.1863-2378.2008.01190.x.
Feces of 70 diarrhoeic and 230 non-diarrhoeic domestic cats from Sao Paulo, Brazil were investigated for enteropathogenic (EPEC), enterohaemorrhagic (EHEC) and enterotoxigenic (ETEC) Escherichia coli types. While ETEC and EHEC strains were not found, 15 EPEC strains were isolated from 14 cats, of which 13 were non-diarrhoeic, and one diarrhoeic. None of 15 EPEC strains carried the bfpA gene or the EPEC adherence factor plasmid, indicating atypical EPEC types. The EPEC strains were heterogeneous with regard to intimin types, such as eae-theta (three strains), eae-kappa (n = 3), eae-alpha1 (n = 2), eae-iota (n = 2), one eae-alpha2, eae-beta1 and eae-eta each, and two were not typeable. The majority of the EPEC isolates adhered to HEp-2 cells in a localized adherence-like pattern and were positive for fluorescence actin staining. The EPEC strains belonged to 12 different serotypes, including O111:H25 and O125:H6, which are known to be pathogens in humans. Multi locus sequence typing revealed a close genetic similarity between the O111:H25 and O125:H6 strains from cats, dogs and humans. Our results show that domestic cats are colonized by EPEC, including serotypes previously described as human pathogens. As these EPEC strains are also isolated from humans, a cycle of mutual infection by EPEC between cats and its households cannot be ruled out, though the transmission dynamics among the reservoirs are not yet understood clearly.
对来自巴西圣保罗的70只腹泻家猫和230只非腹泻家猫的粪便进行了调查，以检测其中的肠致病性大肠杆菌（EPEC）、肠出血性大肠杆菌（EHEC）和产肠毒素大肠杆菌（ETEC）类型。虽然未发现ETEC和EHEC菌株，但从14只猫中分离出了15株EPEC菌株，其中13只猫未腹泻，1只腹泻。15株EPEC菌株均未携带bfpA基因或EPEC黏附因子质粒，表明为非典型EPEC类型。这些EPEC菌株在紧密黏附素类型方面具有异质性，如eae-theta（3株）、eae-kappa（n = 3）、eae-alpha1（n = 2）、eae-iota（n = 2），各有1株eae-alpha2、eae-beta1和eae-eta，还有2株无法分型。大多数EPEC分离株以局部黏附样模式黏附于HEp-2细胞，且荧光肌动蛋白染色呈阳性。这些EPEC菌株属于12种不同的血清型，包括已知的人类病原体O111:H25和O125:H6。多位点序列分型显示，猫、狗和人类的O111:H25和O125:H6菌株之间存在密切的遗传相似性。我们的结果表明，家猫被EPEC定植，包括先前被描述为人类病原体的血清型。由于这些EPEC菌株也从人类中分离出来，尽管尚未清楚了解宿主之间的传播动态，但不能排除猫及其家庭成员之间EPEC相互感染的循环。
