Gu Yu-Huan, Belury Martha A
Bastyr University, 14500 Juanita Drive NE, WA 98028, USA.
Cancer Lett. 2005 Mar 18;220(1):21-8. doi: 10.1016/j.canlet.2004.06.037.
The effects of ethanol extracts from four species of mushroom fruiting bodies, mushroom spores and mushroom cultured broth, were assessed for modulation of cell proliferation and apoptosis in murine skin carcinoma cells (CH72) and non-tumorigenic epidermal cells (C50). While extracts from mycelia of Grifola frondosa, Ganoderma lucidum, Hericium erinaceus, or from spores of G. lucidum exerted little, if any, effect on proliferation, the ethanol-soluble extract of Lentinula edodes (L. edodes) significantly decreased cell proliferation of CH72 cells. There were no changes in the proliferative response of the non-tumorigenic keratinocyte cell line, C50, to any of the mushroom extracts tested. To analyze cell proliferation and apoptosis, fluorescent DNA-microscopy with ethidium bromide and acridine orange staining of cells revealed L. edodes reduced cell proliferation and induced apoptosis in time- and dose-dependent manners in carcinoma cells but had no effect in non-tumorigenic cells (C50). Cell cycle analysis demonstrated that L. edodes extract induced a transient G(1) arrest, with no changes observed in the non-tumorigenic cells (C50).
评估了四种蘑菇子实体、蘑菇孢子和蘑菇培养液的乙醇提取物对小鼠皮肤癌细胞（CH72）和非致瘤性表皮细胞（C50）中细胞增殖和凋亡的调节作用。虽然来自云芝、灵芝、猴头菌的菌丝体提取物或灵芝孢子提取物对增殖几乎没有影响，但香菇的乙醇可溶性提取物显著降低了CH72细胞的增殖。非致瘤性角质形成细胞系C50对所测试的任何蘑菇提取物的增殖反应均无变化。为了分析细胞增殖和凋亡，用溴化乙锭和吖啶橙对细胞进行荧光DNA显微镜检查，结果显示香菇能以时间和剂量依赖性方式降低癌细胞的增殖并诱导凋亡，但对非致瘤性细胞（C50）没有影响。细胞周期分析表明，香菇提取物诱导了短暂的G(1)期阻滞，在非致瘤性细胞（C50）中未观察到变化。
