Tedonkeng Pamo E, Tendonkeng F, Kana J R, Khan Payne V, Boukila B, Lemoufouet J, Miegoue E, Nanda A S
Animal Nutrition Laboratory, Department of Animal Production, FASA, University of Dschang, P.O. Box 222, Dschang, Cameroon.
Vet Parasitol. 2005 Mar 31;128(3-4):319-23. doi: 10.1016/j.vetpar.2004.10.022. Epub 2005 Jan 16.
Study on acaricide property of foam soap containing essential oil of Ageratum houstonianum leaves was tested on Rhipicephalus lunulatus. Four doses of the oil (0.00, 0.02, 0.025 and 0.03 microl/g) with four replications for each dose were used in vitro. Each replication consisted of 10 ticks in a Petri dish with filter paper impregnated uniformly with the foam soap on the bottom. The same four doses in three replications were used in vivo. Each replication was made up of 10 naturally ticks infested goats. Results of this study indicate that foam soap containing essential oil of A. houstonianum leaves is toxic to R. lunulatus. The in vitro mortality rate was observed to vary from 0 to 50% on day 8 after treatment with the controls as compared to 95% with the lowest dose (0.02 microl/g) on day 8 and 100% with the highest dose (0.03 microl/g) on day 3. Meanwhile, the in vivo mortality rate was observed to be 23.4% with the control on day 8 after treatment whereas the highest dose killed 95.1% of the ticks by this day. The LD50 of the foam soap containing essential oil of this plant was 0.0259 and 0.0173 microl/g on day 2 after treatment, in the laboratory and on the farm, respectively. This indicates a potentially high efficiency of this medicated soap on this parasite.
对含藿香蓟叶精油的泡沫皂杀螨特性进行了研究，以新月扇头蜱为试验对象。体外试验中使用了该精油的四个剂量（0.00、0.02、0.025和0.03微升/克），每个剂量重复四次。每次重复在一个培养皿中放入10只蜱，培养皿底部铺有均匀浸渍了泡沫皂的滤纸。体内试验中使用了相同的四个剂量，重复三次。每次重复由10只自然感染蜱的山羊组成。本研究结果表明，含藿香蓟叶精油的泡沫皂对新月扇头蜱有毒性。与对照组相比，体外试验在处理后第8天死亡率从0%到50%不等，而最低剂量（0.02微升/克）在第8天死亡率为95%，最高剂量（0.03微升/克）在第3天死亡率为100%。同时，体内试验在处理后第8天对照组死亡率为23.4%，而最高剂量在这天杀死了95.1%的蜱。含该植物精油的泡沫皂在处理后第2天的半数致死剂量在实验室和农场分别为0.0259和0.0173微升/克。这表明这种药皂对该寄生虫可能具有高效性。
