以海湾蟾鱼（Opsanus tau）血清为结合剂的维生素B12室温放射分析。
Room temperature radioassay for B12 with oyster toadfish (Opsanus tau) serum as binder.
作者信息
Ithakissios D S, Kubiatowicz D O, Wicks J H
出版信息
Clin Chem. 1980 Feb;26(2):323-6.
PMID:7188739
Abstract
We describe a vitamin B12 radioassay with oyster toadfish (Opsanus tau) serum in which endogenous serum B12 is released from its protein complexes at room temperature with NaOH at pH 13.6. The radioassay is reproducible (CV 4.9% within assay, 8.4% between assays) and sensitive (10 ng/L) and requires minimum manipulations. Assay accuracy is unaffected by serum protein.
摘要
我们描述了一种使用牡蛎蟾鱼（Opsanus tau）血清的维生素B12放射免疫测定法，其中内源性血清B12在室温下用pH 13.6的NaOH从其蛋白质复合物中释放出来。该放射免疫测定法具有可重复性（批内变异系数4.9%，批间变异系数8.4%）且灵敏（10 ng/L），并且所需操作最少。测定准确性不受血清蛋白的影响。
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