Nakashima M
Department of Conservative Dentistry, Faculty of Dentistry, Kyushu University, Japan.
Oral Surg Oral Med Oral Pathol. 1992 Apr;73(4):484-9. doi: 10.1016/0030-4220(92)90331-j.
The effects of crude bone morphogenetic protein (BMP) derived from bone and dentin matrix on proliferation, production of extracellular matrix, and biologic function of the pulp cell were examined in the primary cell culture from permanent dental pulp. BMP from bone and from dentin matrix stimulated iodine 125-deoxyuridine incorporation in the absence of 10% calf serum. They increased sulfur 35-sulfate incorporation in proliferating stage and had no effects in stationary stage of culture. Alkaline phosphatase activities were inhibited in proliferating, stationary, and multilayered stages of culture. Osteocalcin synthesis was increased in culture treated with BMP from day 2 to day 10. These findings suggest that crude BMP might have mitogenic activity and some role in regulation of differentiation of pulp cells into odontoblasts.
在从恒牙髓组织进行的原代细胞培养中，检测了源自骨和牙本质基质的粗制骨形态发生蛋白（BMP）对牙髓细胞增殖、细胞外基质产生及生物学功能的影响。来自骨和牙本质基质的BMP在无10%小牛血清的情况下刺激了125碘-脱氧尿苷的掺入。它们在增殖期增加了35硫-硫酸盐的掺入，而在培养静止期无此作用。在培养的增殖期、静止期和多层期，碱性磷酸酶活性均受到抑制。从第2天到第10天，用BMP处理的培养物中骨钙素的合成增加。这些发现表明，粗制BMP可能具有促有丝分裂活性，并在调节牙髓细胞向成牙本质细胞分化中发挥一定作用。
