健康与炎症状态下人类牙髓中的铜锌超氧化物歧化酶活性

Copper-zinc superoxide dismutase activity in healthy and inflamed human dental pulp.

作者信息

Varvara G, Traini T, Esposito P, Caputi S, Perinetti G

机构信息

School of Dentistry, Department of Oral Sciences, University G. D'Annunzio, Via dei Vestini, Chieti, Italy.

出版信息

Int Endod J. 2005 Mar;38(3):195-9. doi: 10.1111/j.1365-2591.2005.00936.x.

DOI:10.1111/j.1365-2591.2005.00936.x

PMID:15743423

Abstract

AIM

To examine copper-zinc superoxide dismutase (Cu, Zn-SOD) activity in clinically healthy and symptomatic human dental pulps.

METHODOLOGY

Twenty-five systemically healthy patients, 14 females and 11 males (age: 13.1-34.6 years; mean: 21.7 +/- 6.3), were the source of the pulp tissue. The condition of the pulps was assessed using clinical and radiographic evaluation. The pulp tissue was collected by longitudinally grooving and splitting the teeth (if extracted) or during endodontic treatment, and were age- and sex-matched between the healthy and the irreversible symptomatic pulpitis tissue groups. Cu, Zn-SOD activity was determined through spectrophotometric methods and a Mann-Whitney test assessed the significance of differences between the groups.

RESULTS

The enzyme activities were 144.8 +/- 42.2 and 68.1 +/- 25.0 U mg(-1) in the healthy and irreversible symptomatic pulp tissue, respectively. The difference between the groups was statistically significant (P < 0.001).

CONCLUSIONS

These results demonstrate a potential role for Cu, Zn-SOD during dental pulp inflammation in humans.

摘要

目的

检测临床健康和有症状的人类牙髓中铜锌超氧化物歧化酶（Cu，Zn-SOD）的活性。

方法

25名全身健康的患者，14名女性和11名男性（年龄：13.1 - 34.6岁；平均：21.7±6.3），为牙髓组织的来源。牙髓状况通过临床和影像学评估。牙髓组织通过纵向开槽和劈开牙齿（如果牙齿已拔除）或在根管治疗期间收集，健康组和不可逆症状性牙髓炎组织组之间在年龄和性别上相匹配。通过分光光度法测定Cu，Zn-SOD活性，采用Mann-Whitney检验评估组间差异的显著性。