枯草芽孢杆菌的Rok蛋白可抑制参与细胞表面及细胞外功能的基因。
The Rok protein of Bacillus subtilis represses genes for cell surface and extracellular functions.
作者信息
Albano Mark, Smits Wiep Klaas, Ho Linh T Y, Kraigher Barbara, Mandic-Mulec Ines, Kuipers Oscar P, Dubnau David
机构信息
Public Health Research Institute, Newark, NJ 07103, USA.
出版信息
J Bacteriol. 2005 Mar;187(6):2010-9. doi: 10.1128/JB.187.6.2010-2019.2005.
Abstract
Rok is a repressor of the transcriptional activator ComK and is therefore an important regulator of competence in Bacillus subtilis (T. T. Hoa, P. Tortosa, M. Albano, and D. Dubnau, Mol. Microbiol. 43:15-26, 2002). To address the wider role of Rok in the physiology of B. subtilis, we have used a combination of transcriptional profiling, gel shift experiments, and the analysis of lacZ fusions. We demonstrate that Rok is a repressor of a family of genes that specify membrane-localized and secreted proteins, including a number of genes that encode products with antibiotic activity. We present evidence for the recent introduction of rok into the B. subtilis-Bacillus licheniformis-Bacilllus amyloliquefaciens group by horizontal transmission.
摘要
Rok是转录激活因子ComK的阻遏物，因此是枯草芽孢杆菌感受态的重要调节因子（T. T. Hoa、P. Tortosa、M. Albano和D. Dubnau，《分子微生物学》43：15 - 26，2002年）。为了研究Rok在枯草芽孢杆菌生理学中的更广泛作用，我们结合了转录谱分析、凝胶迁移实验和lacZ融合分析。我们证明Rok是一类指定膜定位和分泌蛋白的基因家族的阻遏物，包括一些编码具有抗生素活性产物的基因。我们提供证据表明rok最近通过水平转移被引入枯草芽孢杆菌 - 地衣芽孢杆菌 - 解淀粉芽孢杆菌菌群。
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