Im Wan-Taek, Yokota Akira, Kim Myung-Kyum, Lee Sung-Taik
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
J Gen Appl Microbiol. 2004 Oct;50(5):249-54. doi: 10.2323/jgam.50.249.
A taxonomic study was carried out on Chj404T, a bacterial strain isolated from a soil sample collected in an industrial stream near the Chung-Ju industrial complex in Korea. The strain was a gram-negative, aerobic, short rod to coccus-shaped bacterium. It grew well on nutrient agar medium and utilized a broad spectrum of carbon sources. The G+C content of the DNA was 67.4 mol% and the major composition of ubiquinone was Q-10. The major fatty acid was C18:1. Comparative 16S rDNA studies showed a clear affiliation of this bacterium to alpha-Proteobacteria. Comparison of phylogenetic data indicated that it was most closely related to Prosthecomicrobium pneumaticum (92.7% similarity in 16S rDNA sequence). Since strain Chj404 is clearly distinct from closely related species, we propose the name Kaistia adipata gen. nov., sp. nov. for this strain Chj404T (=IAM 15023T=KCTC 12095T).
对从韩国忠州工业园区附近一条工业溪流采集的土壤样本中分离出的细菌菌株Chj404T进行了分类学研究。该菌株为革兰氏阴性、需氧菌,形态为短杆状至球菌状。它在营养琼脂培养基上生长良好,能利用多种碳源。DNA的G+C含量为67.4 mol%,主要泛醌组分为Q-10。主要脂肪酸为C18:1。比较16S rDNA研究表明,该细菌明显属于α-变形菌纲。系统发育数据比较表明,它与产气柄杆菌(16S rDNA序列相似度为92.7%)关系最为密切。由于菌株Chj404明显不同于其近缘物种,我们提议将该菌株Chj404T(=IAM 15023T=KCTC 12095T)命名为脂肪凯斯提亚菌(Kaistia adipata),新属,新种。