Hosoya Ryuichi, Hamana Koei, Niitsu Masaru, Itoh Takashi
Gunma University School of Health Sciences, Maebashi, Gunma 371-8514, Japan.
J Gen Appl Microbiol. 2004 Oct;50(5):271-87. doi: 10.2323/jgam.50.271.
Cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by HPLC and GC analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. Spermidine and a quaternary branched penta-amine, N4-bis(aminopropyl)norspermidine, were the major polyamine in Thermocrinis, Hydrogenobacter, Hydrogenobaculum, Aquifex, Persephonella, Sulfurihydrogenibium, Hydrogenothermus, Balnearium and Thermovibrio, located in the order Aquificales. Thermodesulfobacterium and Thermodesulfatator belonging to the order Thermodesulfobacteriales contained another quaternary penta-amine, N4-bis(aminopropyl)spermidine. In the order Thermotogales, Thermotoga contained spermidine, norspermidine, caldopentamine and homocaldopentamine. The latter two linear penta-amines were not found in Marinitoga and Petrotoga. In the order Thermales, Thermus and Marinithermus contained homospermidine, norspermine and the linear penta-amines. Meiothermus lacked penta-amines. Vulcanithermus contained linear penta-amines and hexa-amines but not homospermidine. Oceanithermus contained spermine alone. Within the order Thermoanaerobacteriales, the two quaternary branched penta-amines were found in Thermanaeromonas and Thermoanaerobacter. Caldanaerobacter contained N4-bis(aminopropyl)spermidine. Thermoanaerobacterium lacked penta-amines. Thermaerobacter of the order Clostridiales contained N4-bis(aminopropyl)spermidine and agmatine. Thermosyntropha, Thermanaerovibrio, Thermobrachium ( the order Clostridiales), Sulfobacillus, Alicyclobacillus, Anoxybacillus, Ureibacillus, Thermicanus ( the order Bacillales), Desulfotomaculum, Desulfitobacterium and Pelotomaculum (the family Peptococcaceae) ubiquitously contained spermine. Some thermophiles of Bacillales added linear and branched penta-amines.
通过高效液相色谱（HPLC）和气相色谱（GC）分析，研究了45种嗜热真细菌和8种相关嗜温真细菌的细胞多胺，以探讨多胺分布谱在嗜热和化学分类学方面的意义。亚精胺和一种四级支链五胺，N4 - 双（氨丙基）降亚精胺，是栖热袍菌属、氢杆菌属、氢棒菌属、嗜水气单胞菌属、珀尔塞福涅菌属、硫氢还原菌属、嗜氢菌属、温泉杆菌属和热弧菌属（均属于产水菌目）中的主要多胺。属于脱硫杆菌目的嗜热脱硫杆菌和嗜热脱硫芽孢杆菌含有另一种四级五胺，N4 - 双（氨丙基）亚精胺。在栖热菌目中，嗜热栖热菌含有亚精胺、降亚精胺、热戊胺和高热戊胺。后两种线性五胺在海栖热袍菌和石油栖热袍菌中未发现。在栖热菌目中，嗜热栖热菌和海栖嗜热栖热菌含有高亚精胺、降精胺和线性五胺。嗜热栖热放线菌缺乏五胺。火山栖热菌含有线性五胺和六胺，但不含高亚精胺。海洋栖热菌仅含有精胺。在热厌氧杆菌目中，两种四级支链五胺存在于嗜热厌氧单胞菌和嗜热厌氧杆菌中。嗜热栖热芽孢杆菌含有N4 - 双（氨丙基）亚精胺。嗜热栖热杆菌缺乏五胺。梭菌目的嗜热栖热杆菌含有N4 - 双（氨丙基）亚精胺和胍丁胺。嗜热共生菌、嗜热厌氧弧菌、嗜热栖热杆菌（梭菌目）、硫杆菌属、嗜酸耐热芽孢杆菌属、嗜热栖热芽孢杆菌属、嗜热栖热芽孢杆菌属、嗜热栖热芽孢杆菌属（芽孢杆菌目）、脱硫肠状菌属、脱硫亚硫酸盐还原菌属和肉毒梭菌属（消化球菌科）普遍含有精胺。芽孢杆菌目的一些嗜热菌还含有线性和支链五胺。
