Hamana Koei, Tanaka Takehiko, Hosoya Ryuichi, Niitsu Masaru, Itoh Takashi
Gunma University School of Health Sciences, Showa-machi, Maebashi, Gunma 371-8514, Japan.
J Gen Appl Microbiol. 2003 Oct;49(5):287-93. doi: 10.2323/jgam.49.287.
Cellular polyamines of newly isolated acidophilic, thermophilic and thermoacidophilic archaebacteria were investigated for the chemotaxonomic significance of polyamine distribution profiles. In addition to spermidine, spermine and agmatine, a quaternary branched penta-amine, N(4)-bis(aminopropyl)spermidine, was found in thermophilic Thermococcus waiotapuensis, Thermococcus aegaeus and Pyrococcus glycovorans belonging to the order Thermococcales. An acidophilic euryarchaeon, Ferroplasma acidiphilum located in the order Thermoplasmatales, contained spermidine and agmatine. Norspermidine, spermidine, norspermine and spermine were found in thermoacidophilic Acidilobus aceticus and thermophilic Thermodiscus maritimus located in the order Desulfurococcales, and in thermophilic Pyrobaculum arsenaticum, Pyrobaculum oguniense, Vulcanisaeta distributa and Vulcanisaeta souniana belonging to the order Thermoproteales; however, the four genera differ on their tetra- and penta-amine levels. Thermophilic Staphylothermus hellenicus belonging to Desulfurococcales contained caldopentamine, caldohexamine and N1-acetylcaldopentamine in addition to norspermidine, spermidine and norspermine. This is the first report on the occurrence of acetylated penta-amine in nature.
研究了新分离的嗜酸、嗜热和嗜热嗜酸古细菌的细胞多胺，以探讨多胺分布谱的化学分类学意义。除了亚精胺、精胺和胍丁胺外，在属于热球菌目的嗜热的怀奥塔普热球菌、爱琴海热球菌和嗜糖嗜热栖热菌中发现了一种季铵支链五胺，即N(4)-双(氨丙基)亚精胺。位于热原体目的嗜酸广古菌嗜酸铁原体含有亚精胺和胍丁胺。在属于脱硫球菌目的嗜热嗜酸乙酸嗜酸菌和嗜热的海栖热盘菌中，以及在属于热变形菌目的嗜热的砷酸热棒菌、奥贡热棒菌、分散硫化叶菌和宗氏硫化叶菌中，发现了降亚精胺、亚精胺、降精胺和精胺；然而，这四个属在其四胺和五胺水平上存在差异。属于脱硫球菌目的嗜热的希腊嗜热栖热菌除了含有降亚精胺、亚精胺和降精胺外，还含有热戊胺、热己胺和N1-乙酰热戊胺。这是关于自然界中乙酰化五胺存在的首次报道。
