Wu Yunhua, Hu Shengshui
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Colloids Surf B Biointerfaces. 2005 Apr 10;41(4):299-304. doi: 10.1016/j.colsurfb.2004.12.019.
Colloid Au (Au(nano)) with a diameter of about 20 nm was prepared and used in combination with the multi-wall carbon nanotubes (MWNTs) to modify a gold electrode. Dihexadecylphosphate (DHP) dispersed in Au(nano) aqueous solution was used to solubilize MWNTs. Deposition of Au(nano) on MWNTs was realized as illustrated by TEM micrographs. The DHP formed a network that connected Au(nano) and MWNTs to the gold electrode surface. The Au(nano)-MWNTs-DHP composite film on the gold electrode surface was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammmetry (CV). The composite film modified gold electrode was used to detect cytochrome c and a pair of well-defined redox waves was obtained. It was found that the composite film promoted the redox of horse heart cytochrome c and its effect was developed for the determination of cytochrome c.
制备了直径约为20nm的胶体金（Au（纳米）），并将其与多壁碳纳米管（MWNTs）结合使用以修饰金电极。分散在Au（纳米）水溶液中的磷酸二己酯（DHP）用于溶解MWNTs。如透射电子显微镜照片所示，实现了Au（纳米）在MWNTs上的沉积。DHP形成了一个网络，将Au（纳米）和MWNTs连接到金电极表面。通过电化学阻抗谱（EIS）和循环伏安法（CV）对金电极表面的Au（纳米）-MWNTs-DHP复合膜进行了表征。该复合膜修饰的金电极用于检测细胞色素c，并获得了一对明确的氧化还原波。发现该复合膜促进了马心细胞色素c的氧化还原，其效果被用于细胞色素c的测定。
