Clarkson Kathleen, Kieffer James D, Currie Suzanne
Department of Biology, Mount Allison University, Sackville, NB, Canada E4L 1G7.
Comp Biochem Physiol A Mol Integr Physiol. 2005 Feb;140(2):225-32. doi: 10.1016/j.cbpb.2005.01.009. Epub 2005 Jan 25.
The goal of this study was to examine the cellular response to exhaustive exercise in male and female rainbow trout to determine if HSPs are involved in the early stages of the recovery process. Levels of HSPs and key metabolic parameters were measured in white muscle, heart plasma, and blood plasma throughout 6 h of recovery from exhaustive burst exercise. Plasma creatine kinase (CK) was also quantified as an indicator of exercise-induced tissue damage. The observed trends in ATP and lactate were consistent with established patterns of exhaustion and the beginnings of metabolic recovery. However, no upregulation of hsp70, hsp30, or hsp90 was evident in heart or muscle tissue of males or females, and plasma CK measurements suggest that tissue damage was minimal. Our results indicate that hsp70, hsp30, and hsp90 are not part of the early recovery process from burst exercise in fish, perhaps due to the maintenance of core temperatures as well as a lack of exercise-induced tissue damage.
本研究的目的是检测雄性和雌性虹鳟鱼对力竭运动的细胞反应，以确定热休克蛋白（HSPs）是否参与恢复过程的早期阶段。在从力竭爆发性运动中恢复的6小时内，对白肌、心脏血浆和血浆中的热休克蛋白水平及关键代谢参数进行了测量。血浆肌酸激酶（CK）也被定量作为运动诱导组织损伤的指标。观察到的三磷酸腺苷（ATP）和乳酸的变化趋势与既定的力竭模式及代谢恢复的开始一致。然而，雄性和雌性的心脏或肌肉组织中热休克蛋白70（hsp70）、热休克蛋白30（hsp30）或热休克蛋白90（hsp90）均未明显上调，血浆CK测量结果表明组织损伤最小。我们的结果表明，hsp70、hsp30和hsp90不是鱼类爆发性运动后早期恢复过程的一部分，这可能是由于核心温度的维持以及缺乏运动诱导的组织损伤。
