Antunes-Neto J M F, Toyama M H, Carneiro E M, Boschero A C, Pereira-da-Silva L, Macedo D V
Instituto de Biologia, Departamento de Bioquímica, UNICAMP, Caixa Postal 6109, CEP, 13083-970, Campinas, São Paulo, Brasil.
Stress. 2006 Jun;9(2):107-15. doi: 10.1080/10253890600772211.
A novel method to measure oxidative stress resulting from exhaustive exercise in rats is presented. In this new procedure we evaluated the erythrocyte antioxidant enzymes, catalase (CAT) and glutathione reductase (GR), the plasma oxidative attack markers, reactive carbonyl derivatives (RCD) and thiobarbituric reactive substances (TBARS). Muscular tissue damage was evaluated by monitoring plasma creatine kinase (CK) and plasma taurine (Tau) concentrations. Also, we monitored total sulphydryl groups (TSG) and uric acid (UA), and the level of the 70 kDa heat shock protein (HSP70) in leukocytes as a marker of oxidative stress. In the study we found a correspondence between erythrocyte CAT and GR activities and leukocyte HSP70 levels, principally 3 h after the acute exercise, and this suggested an integrated mechanism of antioxidant defense. The increase in levels of plasma Tau was coincident with the increasing plasma levels of CK and TBARS, principally after two hours of exercise. Thus tissue damage occurred before the expression of any anti-oxidant system markers and the monitoring of Tau, CK or TBARS may be important for the estimation of oxidative stress during exhaustive exercise. Furthermore, the integrated analyses could be of value in a clinical setting to quantify the extent of oxidative stress risk and reduce the need to perform muscle biopsies as a tool of clinical evaluation.
本文介绍了一种测量大鼠力竭运动后氧化应激的新方法。在这个新方法中，我们评估了红细胞抗氧化酶过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）、血浆氧化损伤标志物、活性羰基衍生物（RCD）和硫代巴比妥反应性物质（TBARS）。通过监测血浆肌酸激酶（CK）和血浆牛磺酸（Tau）浓度来评估肌肉组织损伤。此外，我们还监测了总巯基（TSG）和尿酸（UA），以及白细胞中70 kDa热休克蛋白（HSP70）的水平作为氧化应激的标志物。在研究中，我们发现红细胞CAT和GR活性与白细胞HSP70水平之间存在对应关系，主要在急性运动后3小时，这表明存在抗氧化防御的整合机制。血浆Tau水平的升高与血浆CK和TBARS水平的升高同时出现，主要在运动两小时后。因此，组织损伤在任何抗氧化系统标志物表达之前就已发生，监测Tau、CK或TBARS对于评估力竭运动期间的氧化应激可能很重要。此外，综合分析在临床环境中可能有价值，可用于量化氧化应激风险程度，并减少作为临床评估工具进行肌肉活检的必要性。
